Back to Multiple platform build/check report for BioC 3.21:   simplified   long
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This page was generated on 2025-09-01 11:40 -0400 (Mon, 01 Sep 2025).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo1Linux (Ubuntu 24.04.3 LTS)x86_644.5.1 (2025-06-13) -- "Great Square Root" 4824
merida1macOS 12.7.5 Montereyx86_644.5.1 RC (2025-06-05 r88288) -- "Great Square Root" 4606
kjohnson1macOS 13.6.6 Venturaarm644.5.1 Patched (2025-06-14 r88325) -- "Great Square Root" 4547
kunpeng2Linux (openEuler 24.03 LTS)aarch64R Under development (unstable) (2025-02-19 r87757) -- "Unsuffered Consequences" 4579
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 1503/2341HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
ORFik 1.28.2  (landing page)
Haakon Tjeldnes
Snapshot Date: 2025-08-28 13:40 -0400 (Thu, 28 Aug 2025)
git_url: https://git.bioconductor.org/packages/ORFik
git_branch: RELEASE_3_21
git_last_commit: 87fcbbf
git_last_commit_date: 2025-05-06 04:52:16 -0400 (Tue, 06 May 2025)
nebbiolo1Linux (Ubuntu 24.04.3 LTS) / x86_64  OK    OK    OK  UNNEEDED, same version is already published
merida1macOS 12.7.5 Monterey / x86_64  OK    OK    ERROR    OK  
kjohnson1macOS 13.6.6 Ventura / arm64  OK    OK    ERROR    OK  
kunpeng2Linux (openEuler 24.03 LTS) / aarch64  OK    OK    OK  


CHECK results for ORFik on merida1

To the developers/maintainers of the ORFik package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/ORFik.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: ORFik
Version: 1.28.2
Command: /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:ORFik.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings ORFik_1.28.2.tar.gz
StartedAt: 2025-08-29 07:33:32 -0400 (Fri, 29 Aug 2025)
EndedAt: 2025-08-29 07:52:58 -0400 (Fri, 29 Aug 2025)
EllapsedTime: 1166.4 seconds
RetCode: 1
Status:   ERROR  
CheckDir: ORFik.Rcheck
Warnings: NA

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:ORFik.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings ORFik_1.28.2.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/Users/biocbuild/bbs-3.21-bioc/meat/ORFik.Rcheck’
* using R version 4.5.1 RC (2025-06-05 r88288)
* using platform: x86_64-apple-darwin20
* R was compiled by
    Apple clang version 14.0.0 (clang-1400.0.29.202)
    GNU Fortran (GCC) 14.2.0
* running under: macOS Monterey 12.7.6
* using session charset: UTF-8
* using option ‘--no-vignettes’
* checking for file ‘ORFik/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘ORFik’ version ‘1.28.2’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... INFO
Imports includes 28 non-default packages.
Importing from so many packages makes the package vulnerable to any of
them becoming unavailable.  Move as many as possible to Suggests and
use conditionally.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... NOTE
Found the following non-portable file path:
  ORFik/inst/extdata/test_processing/contaminants_depletion/LOGS/contaminants_output_template_Log.final.out

Tarballs are only required to store paths of up to 100 bytes and cannot
store those of more than 256 bytes, with restrictions including to 100
bytes for the final component.
See section ‘Package structure’ in the ‘Writing R Extensions’ manual.
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘ORFik’ can be installed ... OK
* used C++ compiler: ‘Apple clang version 14.0.0 (clang-1400.0.29.202)’
* used SDK: ‘MacOSX11.3.sdk’
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... NOTE
License stub is invalid DCF.
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... NOTE
Unexported objects imported by ':::' calls:
  ‘GenomicFeatures:::.merge_seqinfo_and_infer_missing_seqlengths’
  ‘IRanges:::regroupBySupergroup’ ‘S4Vectors:::normarg_mcols’
  ‘biomartr:::getENSEMBL.Seq’ ‘biomartr:::getENSEMBL.gtf’
  See the note in ?`:::` about the use of this operator.
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
DEG.plot.static: no visible binding for global variable ‘Regulation’
DEG.plot.static: no visible binding for global variable ‘meanCounts’
DEG.plot.static: no visible binding for global variable ‘LFC’
DEG_model_simple: no visible binding for global variable ‘id’
DEG_model_simple: no visible binding for global variable ‘contrast’
DTEG.plot: no visible binding for global variable ‘Regulation’
DTEG.plot: no visible binding for global variable ‘rna.lfc’
DTEG.plot: no visible binding for global variable ‘rfp.lfc’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘te.sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘rfp.sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘rna.sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘Regulation’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘all_models_sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘te.lfc’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘rna.lfc’
DTEG_pair_results: no visible binding for global variable ‘rna.sign’
DTEG_pair_results: no visible binding for global variable ‘rna.padj’
DTEG_pair_results: no visible binding for global variable ‘rfp.sign’
DTEG_pair_results: no visible binding for global variable ‘rfp.padj’
DTEG_pair_results: no visible binding for global variable ‘te.sign’
DTEG_pair_results: no visible binding for global variable ‘te.padj’
DTEG_pair_results: no visible binding for global variable
  ‘all_models_sign’
QCstats.plot: no visible binding for global variable ‘variable’
QCstats.plot: no visible binding for global variable ‘sample_total’
QCstats.plot: no visible binding for global variable ‘value’
QCstats.plot: no visible global function definition for ‘.’
QCstats.plot: no visible binding for global variable ‘sample_id’
QCstats.plot: no visible binding for global variable ‘percentage’
QCstats.plot: no visible binding for global variable
  ‘perc_of_counts_per_sample’
QCstats.plot: no visible binding for global variable ‘read length’
RiboQC.plot: no visible binding for global variable ‘variable’
RiboQC.plot: no visible binding for global variable ‘sample_total’
RiboQC.plot: no visible binding for global variable ‘value’
RiboQC.plot: no visible global function definition for ‘.’
RiboQC.plot: no visible binding for global variable ‘sample_id’
RiboQC.plot: no visible binding for global variable ‘percentage’
RiboQC.plot: no visible binding for global variable ‘percent’
RiboQC.plot: no visible binding for global variable ‘frame’
STAR.multiQC_plot: no visible binding for global variable ‘value’
STAR.multiQC_plot: no visible binding for global variable ‘sample_id’
TOP.Motif.ecdf: no visible binding for global variable ‘seq1’
TOP.Motif.ecdf: no visible binding for global variable ‘TOP’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘percentage_mrna_aligned’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘mRNA’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘percentage_tx_aligned’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘Transcript’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘ratio_cds_mrna’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘CDS’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘ratio_cds_leader’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘LEADERS’
allFeaturesHelper: no visible binding for global variable ‘te’
allFeaturesHelper: no visible binding for global variable ‘fpkmRFP’
allFeaturesHelper: no visible binding for global variable ‘fpkmRNA’
allFeaturesHelper: no visible binding for global variable ‘countRFP’
allFeaturesHelper: no visible binding for global variable ‘entropyRFP’
allFeaturesHelper: no visible binding for global variable
  ‘disengagementScores’
allFeaturesHelper: no visible binding for global variable ‘RRS’
allFeaturesHelper: no visible binding for global variable ‘RSS’
allFeaturesHelper: no visible binding for global variable ‘ORFScores’
allFeaturesHelper: no visible binding for global variable ‘ioScore’
allFeaturesHelper: no visible binding for global variable
  ‘startCodonCoverage’
allFeaturesHelper: no visible binding for global variable
  ‘startRegionRelative’
allFeaturesHelper: no visible binding for global variable ‘kozak’
allFeaturesHelper: no visible binding for global variable ‘StartCodons’
allFeaturesHelper: no visible binding for global variable ‘StopCodons’
allFeaturesHelper: no visible binding for global variable
  ‘fractionLengths’
allFeaturesHelper: no visible binding for global variable ‘distORFCDS’
allFeaturesHelper: no visible binding for global variable ‘inFrameCDS’
allFeaturesHelper: no visible binding for global variable
  ‘isOverlappingCds’
allFeaturesHelper: no visible binding for global variable ‘rankInTx’
appendZeroes: no visible binding for global variable ‘frame’
appendZeroes: no visible binding for global variable ‘position’
appendZeroes: no visible binding for global variable ‘count’
artificial.orfs: no visible binding for global variable ‘random’
artificial.orfs: no visible binding for global variable ‘pick’
artificial.orfs: no visible global function definition for ‘.’
browseSRA: no visible global function definition for ‘browseURL’
cellTypeNames: missing arguments not allowed in calls to ‘c’
codonSumsPerGroup: no visible binding for global variable ‘genes’
codon_usage: no visible binding for global variable ‘merged’
codon_usage: no visible binding for global variable ‘AA’
codon_usage: no visible binding for global variable ‘codon’
codon_usage_plot: no visible binding for global variable ‘seqs’
collapse.by.scores: no visible global function definition for ‘.’
collapse.fastq.internal: no visible binding for global variable ‘N’
cor_plot: no visible binding for global variable ‘Var2’
cor_plot: no visible binding for global variable ‘Var1’
cor_plot: no visible binding for global variable ‘Cor’
cor_table: no visible binding for global variable ‘Var1’
cor_table: no visible binding for global variable ‘Var2’
cor_table: no visible binding for global variable ‘Cor’
coverageHeatMap: no visible binding for global variable ‘position’
coverageHeatMap: no visible binding for global variable ‘fraction’
coveragePerORFStatistics: no visible global function definition for ‘.’
coveragePerORFStatistics: no visible binding for global variable
  ‘count’
coveragePerORFStatistics: no visible binding for global variable
  ‘genes’
coverageScorings: no visible binding for global variable ‘count’
coverageScorings: no visible binding for global variable ‘zscore’
coverageScorings: no visible binding for global variable ‘windowMean’
coverageScorings: no visible binding for global variable ‘windowSD’
coverageScorings: no visible global function definition for ‘.’
coverageScorings: no visible binding for global variable ‘modzscore’
coverageScorings: no visible binding for global variable ‘windowMedian’
coverageScorings: no visible binding for global variable ‘windowMAD’
coverageScorings: no visible binding for global variable ‘gene_sum’
coverageScorings: no visible binding for global variable ‘fraction’
coverage_random_access_file: no visible binding for global variable
  ‘genes’
coverage_random_access_file: no visible binding for global variable
  ‘position’
coverage_random_access_file: no visible binding for global variable
  ‘frame’
coverage_to_dt: no visible binding for global variable ‘frame’
coverage_to_dt: no visible binding for global variable ‘genes’
coverage_to_dt: no visible binding for global variable ‘position’
detectRibosomeShifts: no visible global function definition for ‘.’
detectRibosomeShifts: no visible binding for global variable ‘size’
detectRibosomeShifts: no visible binding for global variable ‘fraction’
detectRibosomeShifts: no visible binding for global variable ‘pShifted’
detectRibosomeShifts: no visible binding for global variable
  ‘sum.count’
detectRibosomeShifts: no visible binding for global variable ‘count’
detectRibosomeShifts: no visible binding for global variable ‘genes’
detectRibosomeShifts: no visible binding for global variable
  ‘frac.score’
entropy: no visible binding for global variable ‘Hx’
entropy: no visible binding for global variable ‘codonSums’
entropy: no visible global function definition for ‘.’
entropy: no visible binding for global variable ‘genes’
fetch_xml_attributes: no visible binding for global variable
  ‘..to_keep’
filterExtremePeakGenes: no visible binding for global variable ‘count’
filterExtremePeakGenes: no visible binding for global variable
  ‘median_per_gene’
filterExtremePeakGenes: no visible binding for global variable ‘genes’
filterExtremePeakGenes: no visible global function definition for ‘.’
filterTranscripts: no visible binding for global variable ‘utr5_len’
filterTranscripts: no visible binding for global variable ‘utr3_len’
filter_empty_runs: no visible binding for global variable ‘spots’
findNGSPairs: no visible global function definition for ‘.’
findNGSPairs: no visible binding for global variable ‘forward’
findPeaksPerGene: no visible binding for global variable ‘sum_per_gene’
findPeaksPerGene: no visible binding for global variable ‘count’
findPeaksPerGene: no visible binding for global variable ‘genes’
findPeaksPerGene: no visible binding for global variable
  ‘mean_per_gene’
findPeaksPerGene: no visible binding for global variable
  ‘median_per_gene’
findPeaksPerGene: no visible binding for global variable ‘sd_per_gene’
findPeaksPerGene: no visible binding for global variable ‘mad_per_gene’
findPeaksPerGene: no visible binding for global variable ‘zscore’
findPeaksPerGene: no visible binding for global variable ‘modzscore’
findPeaksPerGene: no visible binding for global variable ‘gene_id’
find_url_ebi_safe: no visible binding for global variable
  ‘run_accession’
floss: no visible binding for global variable ‘ORFGrouping’
floss: no visible binding for global variable ‘widths’
floss: no visible global function definition for ‘.’
floss: no visible binding for global variable ‘CDSGrouping’
floss: no visible binding for global variable ‘fraction.x’
floss: no visible binding for global variable ‘fraction.y’
gSort: no visible binding for global variable ‘grnames’
geneToSymbol: no visible binding for global variable ‘ensembl_gene_id’
geneToSymbol: no visible binding for global variable
  ‘external_gene_name’
geneToSymbol: no visible binding for global variable
  ‘ensembl_transcript_id’
geneToSymbol: no visible binding for global variable ‘uniprotswissprot’
getNGenesCoverage: no visible global function definition for ‘.’
getNGenesCoverage: no visible binding for global variable ‘genes’
getNGenesCoverage: no visible binding for global variable ‘fraction’
get_phix_genome: no visible binding for global variable ‘phix.url’
grl_has_any_valid_lengths: no visible binding for global variable ‘grl’
initiationScore: no visible global function definition for ‘.’
initiationScore: no visible binding for global variable ‘dif’
initiationScore: no visible binding for global variable ‘fraction’
initiationScore: no visible binding for global variable ‘genes’
initiationScore: no visible binding for global variable ‘difPer’
install.sratoolkit: no visible binding for global variable ‘osVersion’
isPeriodic: no visible binding for global variable ‘spec’
kozakHeatmap: no visible global function definition for ‘.’
kozakHeatmap: no visible binding for global variable ‘variable’
kozakHeatmap: no visible binding for global variable ‘value’
kozakHeatmap: no visible binding for global variable
  ‘count_seq_pos_with_count’
kozakHeatmap: no visible binding for global variable ‘median_score’
kozak_IR_ranking: no visible global function definition for ‘.’
kozak_IR_ranking: no visible binding for global variable ‘IR’
kozak_IR_ranking: no visible binding for global variable
  ‘upstream_kozak_strength’
kozak_IR_ranking: no visible binding for global variable ‘count’
kozak_IR_ranking: no visible binding for global variable ‘mean_IR’
list.genomes: no visible binding for global variable ‘STAR_index’
longestORFs: no visible global function definition for ‘.’
metaWindow: no visible binding for global variable ‘position’
metaWindow: no visible binding for global variable ‘frame’
metadata.autnaming: no visible binding for global variable
  ‘LIBRARYTYPE’
metadata.autnaming: no visible binding for global variable
  ‘LibraryStrategy’
ofst_merge_internal: no visible binding for global variable
  ‘file_paths’
orfFrameDistributions : <anonymous>: no visible binding for global
  variable ‘fraction’
orfFrameDistributions: no visible binding for global variable ‘percent’
orfFrameDistributions: no visible binding for global variable
  ‘fraction’
orfFrameDistributions: no visible binding for global variable
  ‘percent_length’
orfFrameDistributions: no visible global function definition for ‘.’
orfFrameDistributions: no visible binding for global variable
  ‘best_frame’
orfScore: no visible binding for global variable ‘genes’
orfScore: no visible binding for global variable ‘frame’
orfScore: no visible binding for global variable ‘frame_one_RP’
orfScore: no visible binding for global variable ‘frame_two_RP’
pSitePlot: no visible binding for global variable ‘count’
pSitePlot: no visible binding for global variable ‘frame’
pSitePlot: no visible binding for global variable ‘position’
readBam: no visible global function definition for ‘bamTag<-’
readBam: no visible global function definition for ‘bamWhat<-’
readLengthTable: no visible binding for global variable
  ‘counts_per_sample’
readLengthTable: no visible binding for global variable ‘sample_id’
readLengthTable: no visible binding for global variable
  ‘perc_of_counts_per_sample’
regionPerReadLength : <anonymous>: no visible binding for global
  variable ‘fraction’
remakeTxdbExonIds: no visible global function definition for ‘.’
remakeTxdbExonIds: no visible binding for global variable ‘chr’
removeTxdbExons: no visible binding for global variable ‘exon_rank’
removeTxdbExons: no visible binding for global variable ‘ranks’
ribo_fft: no visible binding for global variable ‘fraction’
ribo_fft_plot: no visible binding for global variable ‘periods’
ribo_fft_plot: no visible binding for global variable ‘amplitude’
rnaNormalize: no visible binding for global variable ‘genes’
rnaNormalize: no visible binding for global variable ‘feature’
sample_info_append_SRA: no visible binding for global variable ‘GEO’
scaledWindowPositions: no visible binding for global variable
  ‘scalingFactor’
scaledWindowPositions: no visible binding for global variable ‘genes’
scaledWindowPositions: no visible binding for global variable
  ‘position’
scaledWindowPositions: no visible global function definition for ‘.’
scoreSummarizedExperiment: no visible global function definition for
  ‘rowSums2’
seq_usage: no visible binding for global variable ‘variable’
seq_usage: no visible binding for global variable ‘codon_sum’
seq_usage: no visible global function definition for ‘.’
seq_usage: no visible binding for global variable ‘gene_sum’
seq_usage: no visible binding for global variable
  ‘N_AA_of_type_per_gene’
seq_usage: no visible binding for global variable ‘as_prob_normalized’
seq_usage: no visible binding for global variable ‘N_total’
seq_usage: no visible binding for global variable ‘N’
seq_usage: no visible binding for global variable ‘mean_txNorm’
seq_usage: no visible binding for global variable ‘sum_txNorm’
seq_usage: no visible binding for global variable ‘dispersion’
seq_usage: no visible binding for global variable ‘dispersion_txNorm’
seq_usage: no visible binding for global variable ‘var_txNorm’
seq_usage: no visible binding for global variable ‘mean_percentage’
seq_usage: no visible binding for global variable ‘mean_txNorm_prob’
seq_usage: no visible binding for global variable
  ‘mean_txNorm_percentage’
seq_usage: no visible binding for global variable
  ‘relative_to_max_score’
shiftPlots : <anonymous>: no visible binding for global variable
  ‘frame’
shiftPlots : <anonymous>: no visible binding for global variable
  ‘position’
te.plot: no visible global function definition for ‘rowMin’
te.plot: no visible binding for global variable ‘variable’
te.plot: no visible binding for global variable ‘LFC_TE’
te.plot: no visible binding for global variable ‘rfp_log2’
te.plot: no visible binding for global variable ‘rna_log2’
te.plot: no visible binding for global variable ‘rna_log10’
te.table: no visible global function definition for ‘rowMin’
te.table: no visible binding for global variable ‘variable’
te.table: no visible binding for global variable ‘TE_log2’
te.table: no visible binding for global variable ‘rfp_log2’
te.table: no visible binding for global variable ‘rna_log2’
te_rna.plot: no visible binding for global variable ‘subtitle’
te_rna.plot: no visible binding for global variable ‘rna_log10’
te_rna.plot: no visible binding for global variable ‘TE_log2’
topMotif: no visible binding for global variable ‘seq1’
topMotif: no visible binding for global variable ‘seq2’
topMotif: no visible binding for global variable ‘seq3’
topMotif: no visible binding for global variable ‘seq4’
topMotif: no visible binding for global variable ‘seq5’
transcriptWindow: no visible binding for global variable ‘fractions’
transcriptWindow: no visible binding for global variable ‘feature’
unlistToExtremities: no visible global function definition for ‘.’
unlistToExtremities: no visible binding for global variable ‘group’
windowCoveragePlot: no visible binding for global variable ‘feature’
windowCoveragePlot: no visible binding for global variable ‘fraction’
windowCoveragePlot: no visible binding for global variable
  ‘fraction_min’
windowCoveragePlot: no visible binding for global variable ‘position’
collapseDuplicatedReads,GAlignmentPairs: no visible global function
  definition for ‘.’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘start1’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘start2’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘cigar1’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘cigar2’
collapseDuplicatedReads,GAlignments: no visible global function
  definition for ‘.’
collapseDuplicatedReads,GRanges: no visible binding for global variable
  ‘size’
collapseDuplicatedReads,GRanges: no visible global function definition
  for ‘.’
collapseDuplicatedReads,data.table: no visible global function
  definition for ‘.’
design,experiment: no visible binding for global variable ‘..formula’
seqlevels,experiment: no visible binding for global variable ‘df’
show,covRleList: no visible global function definition for ‘head’
Undefined global functions or variables:
  . ..formula ..to_keep AA CDS CDSGrouping Cor GEO Hx IR LEADERS LFC
  LFC_TE LIBRARYTYPE LibraryStrategy N N_AA_of_type_per_gene N_total
  ORFGrouping ORFScores RRS RSS Regulation STAR_index StartCodons
  StopCodons TE_log2 TOP Transcript Var1 Var2 all_models_sign amplitude
  as_prob_normalized bamTag<- bamWhat<- best_frame browseURL chr cigar1
  cigar2 codon codonSums codon_sum contrast count countRFP
  count_seq_pos_with_count counts_per_sample df dif difPer
  disengagementScores dispersion dispersion_txNorm distORFCDS
  ensembl_gene_id ensembl_transcript_id entropyRFP exon_rank
  external_gene_name feature file_paths forward fpkmRFP fpkmRNA
  frac.score fraction fraction.x fraction.y fractionLengths
  fraction_min fractions frame frame_one_RP frame_two_RP gene_id
  gene_sum genes grl grnames group head id inFrameCDS ioScore
  isOverlappingCds kozak mRNA mad_per_gene meanCounts mean_IR
  mean_per_gene mean_percentage mean_txNorm mean_txNorm_percentage
  mean_txNorm_prob median_per_gene median_score merged modzscore
  osVersion pShifted perc_of_counts_per_sample percent percent_length
  percentage percentage_mrna_aligned percentage_tx_aligned periods
  phix.url pick position random rankInTx ranks ratio_cds_leader
  ratio_cds_mrna read length relative_to_max_score rfp.lfc rfp.padj
  rfp.sign rfp_log2 rna.lfc rna.padj rna.sign rna_log10 rna_log2 rowMin
  rowSums2 run_accession sample_id sample_total scalingFactor
  sd_per_gene seq1 seq2 seq3 seq4 seq5 seqs size spec spots start1
  start2 startCodonCoverage startRegionRelative subtitle sum.count
  sum_per_gene sum_txNorm te te.lfc te.padj te.sign uniprotswissprot
  upstream_kozak_strength utr3_len utr5_len value var_txNorm variable
  widths windowMAD windowMean windowMedian windowSD zscore
Consider adding
  importFrom("base", "length")
  importFrom("graphics", "frame")
  importFrom("stats", "df")
  importFrom("utils", "browseURL", "head", "osVersion")
to your NAMESPACE file.
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... NOTE
Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  DTEG.analysis.Rd: SummarizedExperiment
  ORFikQC.Rd: SummarizedExperiment
  QCreport.Rd: SummarizedExperiment
  allFeaturesHelper.Rd: GRangesList, GAlignments, GRanges, FaFile
  asTX.Rd: GRangesList
  assignFirstExonsStartSite.Rd: GRangesList
  assignLastExonsStopSite.Rd: GRangesList
  bedToGR.Rd: GRanges
  codonSumsPerGroup.Rd: GRangesList, GAlignments, GRanges
  codon_usage_exp.Rd: FaFile
  computeFeatures.Rd: GRangesList, GAlignments, GRanges, FaFile
  computeFeaturesCage.Rd: GRangesList, GAlignments, GRanges, FaFile
  coverageByTranscriptC.Rd: coverageByTranscript, GRangesList
  coverageByTranscriptW.Rd: coverageByTranscript, GRanges, GAlignments,
    GRangesList
  coveragePerTiling.Rd: GRangesList, GAlignments, GRanges
  detectRibosomeShifts.Rd: GAlignments
  disengagementScore.Rd: GRangesList, TxDb
  distToCds.Rd: GRangesList
  distToTSS.Rd: GRangesList
  downstreamFromPerGroup.Rd: GRangesList
  downstreamOfPerGroup.Rd: GRangesList
  entropy.Rd: GRangesList, GRanges, GAlignments
  experiment-class.Rd: SummarizedExperiment
  export.ofst-GAlignmentPairs-method.Rd: fst-package
  export.ofst-GAlignments-method.Rd: fst-package
  export.ofst-GRanges-method.Rd: fst-package
  export.ofst.Rd: fst-package
  extendLeaders.Rd: GRangesList
  extendTrailers.Rd: GRangesList
  fimport.Rd: seqlevelsStyle, Seqinfo, GAlignments, GRanges
  findFa.Rd: FaFile
  findMapORFs.Rd: GRangesList, FaFile
  findORFs.Rd: IRanges, IRangesList, FaFile
  findUORFs.Rd: FaFile
  firstEndPerGroup.Rd: GRangesList
  firstExonPerGroup.Rd: GRangesList
  firstStartPerGroup.Rd: GRangesList
  flankPerGroup.Rd: GRangesList
  floss.Rd: GRangesList, GRanges, GAlignments
  fpkm.Rd: GRangesList, GRanges, GAlignments
  fractionLength.Rd: GRangesList
  fread.bed.Rd: import.bed, seqlevelsStyle, Seqinfo, GRanges
  gSort.Rd: GRangesList
  hasHits.Rd: GRangesList
  heatMapL.Rd: GRangesList
  heatMapRegion.Rd: GRanges, GAlignments
  heatMap_single.Rd: GRangesList, GAlignments, GRanges
  import.ofst.Rd: fst-package, GAlignmentPairs
  initiationScore.Rd: GRangesList, GAlignments
  insideOutsideORF.Rd: GRangesList, TxDb
  kozakSequenceScore.Rd: GRangesList, FaFile
  kozak_IR_ranking.Rd: FaFile
  lastExonEndPerGroup.Rd: GRangesList
  lastExonPerGroup.Rd: GRangesList
  lastExonStartPerGroup.Rd: GRangesList
  loadTxdb.Rd: seqlevelsStyle, Seqinfo
  longestORFs.Rd: GRangesList
  makeExonRanks.Rd: GRangesList
  makeORFNames.Rd: GRangesList
  makeSummarizedExperimentFromBam.Rd: GRangesList, SummarizedExperiment
  mapToGRanges.Rd: GRangesList
  matchNaming.Rd: GRangesList
  matchSeqStyle.Rd: seqlevelsStyle, Seqinfo
  numCodons.Rd: GRangesList
  numExonsPerGroup.Rd: GRangesList
  optimizeReads.Rd: GRangesList
  optimizedTranscriptLengths.Rd: transcriptLengths
  orfID.Rd: GRangesList
  orfScore.Rd: GRangesList, GAlignments, GRanges
  outputLibs.Rd: seqlevelsStyle, Seqinfo
  rankOrder.Rd: GRangesList
  readBam.Rd: readGAlignments, seqlevelsStyle, Seqinfo, GAlignments,
    GAlignmentPairs
  readBigWig.Rd: seqlevelsStyle, Seqinfo, GRanges
  readWig.Rd: seqlevelsStyle, Seqinfo, GRanges
  reduceKeepAttr.Rd: GRangesList, reduce
  regionPerReadLength.Rd: GRangesList, GAlignments, GRanges
  reverseMinusStrandPerGroup.Rd: GRangesList
  ribo_fft.Rd: GAlignments, GRanges, GRangesList
  ribosomeReleaseScore.Rd: GRangesList, GAlignments, GRanges
  ribosomeStallingScore.Rd: GRangesList
  rnaNormalize.Rd: GRangesList
  scaledWindowPositions.Rd: GRangesList, GAlignments, GRanges
  seqnamesPerGroup.Rd: GRangesList
  shiftFootprints.Rd: GAlignments, GRanges
  sortPerGroup.Rd: sort.GenomicRanges, GRangesList
  splitIn3Tx.Rd: GRangesList
  startCodons.Rd: GRangesList
  startRegion.Rd: GRangesList
  startRegionCoverage.Rd: GRangesList
  startRegionString.Rd: GRangesList, FaFile
  startSites.Rd: GRangesList
  stopCodons.Rd: GRangesList
  stopRegion.Rd: GRangesList
  stopSites.Rd: GRangesList
  strandBool.Rd: GRangesList
  strandPerGroup.Rd: GRangesList
  tile1.Rd: GRangesList
  transcriptWindow.Rd: GRangesList
  transcriptWindowPer.Rd: GRangesList
  translationalEff.Rd: GRangesList, GRanges, GAlignments
  txNames.Rd: GRangesList
  txSeqsFromFa.Rd: GRangesList, FaFile, extractTranscriptSeqs,
    DNAStringSet
  uniqueGroups.Rd: GRangesList
  uniqueOrder.Rd: GRangesList, GRanges
  unlistToExtremities.Rd: GRangesList
  upstreamFromPerGroup.Rd: GRangesList
  upstreamOfPerGroup.Rd: GRangesList
  validSeqlevels.Rd: GRangesList
  widthPerGroup.Rd: GRangesList
  windowPerGroup.Rd: GRangesList
  windowPerReadLength.Rd: GRangesList, GAlignments, GRanges
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking compiled code ... NOTE
Note: information on .o files is not available
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘ORFik-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: get_system_usage
> ### Title: System usage for Linux (Auto-detects correct drive if not
> ###   provided)
> ### Aliases: get_system_usage
> 
> ### ** Examples
> 
> get_system_usage()
invalid option or syntax: -bn1
top usage: top
		[-a | -d | -e | -c <mode>]
		[-F | -f]
		[-h]
		[-i <interval>]
		[-l <samples>]
		[-ncols <columns>]
		[-o <key>] [-O <secondaryKey>]
			keys: pid (default), command, cpu, cpu_me, cpu_others, csw,
				time, threads, ports, mregion, mem, rprvt, purg, vsize, vprvt,
				kprvt, kshrd, pgrp, ppid, state, uid, wq, faults, cow, user,
				msgsent, msgrecv, sysbsd, sysmach, pageins, boosts, instrs, cycles
		[-R | -r]
		[-S]
		[-s <delay>]
		[-n <nprocs>]
		[-stats <key(s)>]
		[-pid <processid>]
		[-user <username>]
		[-U <username>]
		[-u]

sh: free: command not found
Error in strsplit(mem_info, " ")[[1]] : subscript out of bounds
Calls: get_system_usage
Execution halted
Examples with CPU (user + system) or elapsed time > 5s
                        user system elapsed
codon_usage_exp       16.699  0.749  17.966
STAR.allsteps.multiQC 15.906  0.107  16.150
detect_ribo_orfs      15.089  0.853  16.101
findUORFs_exp         11.246  0.069  11.639
computeFeatures        9.534  0.109  10.061
codon_usage_plot       7.838  0.828   9.003
codon_usage            7.193  0.065   7.381
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 1 ERROR, 6 NOTEs
See
  ‘/Users/biocbuild/bbs-3.21-bioc/meat/ORFik.Rcheck/00check.log’
for details.


Installation output

ORFik.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL ORFik
###
##############################################################################
##############################################################################


* installing to library ‘/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library’
* installing *source* package ‘ORFik’ ...
** this is package ‘ORFik’ version ‘1.28.2’
** using staged installation
** libs
using C++ compiler: ‘Apple clang version 14.0.0 (clang-1400.0.29.202)’
using SDK: ‘MacOSX11.3.sdk’
clang++ -arch x86_64 -std=gnu++17 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG  -I'/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/Rcpp/include' -I/opt/R/x86_64/include    -fPIC  -falign-functions=64 -Wall -g -O2   -c RcppExports.cpp -o RcppExports.o
clang++ -arch x86_64 -std=gnu++17 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG  -I'/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/Rcpp/include' -I/opt/R/x86_64/include    -fPIC  -falign-functions=64 -Wall -g -O2   -c findORFsHelpers.cpp -o findORFsHelpers.o
clang++ -arch x86_64 -std=gnu++17 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG  -I'/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/Rcpp/include' -I/opt/R/x86_64/include    -fPIC  -falign-functions=64 -Wall -g -O2   -c findOrfs.cpp -o findOrfs.o
clang++ -arch x86_64 -std=gnu++17 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG  -I'/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/Rcpp/include' -I/opt/R/x86_64/include    -fPIC  -falign-functions=64 -Wall -g -O2   -c findOrfsFasta.cpp -o findOrfsFasta.o
clang++ -arch x86_64 -std=gnu++17 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG  -I'/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/Rcpp/include' -I/opt/R/x86_64/include    -fPIC  -falign-functions=64 -Wall -g -O2   -c pmapFromTranscripts.cpp -o pmapFromTranscripts.o
clang++ -arch x86_64 -std=gnu++17 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG  -I'/Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/Rcpp/include' -I/opt/R/x86_64/include    -fPIC  -falign-functions=64 -Wall -g -O2   -c pmapToTranscripts.cpp -o pmapToTranscripts.o
pmapToTranscripts.cpp:22:7: warning: variable 'currentWidth' set but not used [-Wunused-but-set-variable]
  int currentWidth = 0;
      ^
pmapToTranscripts.cpp:68:7: warning: variable 'currentWidth' set but not used [-Wunused-but-set-variable]
  int currentWidth = 0;
      ^
2 warnings generated.
clang++ -arch x86_64 -std=gnu++17 -dynamiclib -Wl,-headerpad_max_install_names -undefined dynamic_lookup -L/Library/Frameworks/R.framework/Resources/lib -L/opt/R/x86_64/lib -o ORFik.so RcppExports.o findORFsHelpers.o findOrfs.o findOrfsFasta.o pmapFromTranscripts.o pmapToTranscripts.o -F/Library/Frameworks/R.framework/.. -framework R
installing to /Library/Frameworks/R.framework/Versions/4.5-x86_64/Resources/library/00LOCK-ORFik/00new/ORFik/libs
** R
** inst
** byte-compile and prepare package for lazy loading
Creating a new generic function for ‘symbols’ in package ‘ORFik’
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** checking absolute paths in shared objects and dynamic libraries
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (ORFik)

Tests output

ORFik.Rcheck/tests/testthat.Rout


R version 4.5.1 RC (2025-06-05 r88288) -- "Great Square Root"
Copyright (C) 2025 The R Foundation for Statistical Computing
Platform: x86_64-apple-darwin20

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(ORFik)
Loading required package: IRanges
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:utils':

    findMatches

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Loading required package: GenomicAlignments
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats

Attaching package: 'MatrixGenerics'

The following objects are masked from 'package:matrixStats':

    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars

Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


Attaching package: 'Biobase'

The following object is masked from 'package:MatrixGenerics':

    rowMedians

The following objects are masked from 'package:matrixStats':

    anyMissing, rowMedians

Loading required package: Biostrings
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

Loading required package: Rsamtools

Attaching package: 'ORFik'

The following object is masked from 'package:graphics':

    symbols

> 
> test_check("ORFik")
Error in x$.self$finalize() : attempt to apply non-function
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 440 ]
Warning message:
call dbDisconnect() when finished working with a connection 
> 
> proc.time()
   user  system elapsed 
203.958   6.239 213.591 

Example timings

ORFik.Rcheck/ORFik-Ex.timings

nameusersystemelapsed
DEG.analysis1.4440.0441.554
DEG.plot.static1.0570.0151.101
DEG_model2.1400.0392.250
DEG_model_results1.0690.0071.089
DEG_model_simple1.0850.1121.212
DTEG.analysis1.1540.1411.373
DTEG.plot1.0920.0711.227
ORFik.template.experiment1.1260.0091.168
ORFik.template.experiment.zf0.1400.0030.145
ORFikQC1.0270.0091.055
QCreport1.0100.0091.027
QCstats1.0280.0101.047
QCstats.plot1.0120.0051.023
RiboQC.plot0.9480.0060.955
STAR.align.folder0.0000.0020.003
STAR.align.single0.0010.0010.002
STAR.allsteps.multiQC15.906 0.10716.150
STAR.index0.0000.0010.001
STAR.install0.0010.0010.001
STAR.multiQC4.9310.0194.959
STAR.remove.crashed.genome0.0000.0000.001
TOP.Motif.ecdf0.0010.0000.001
artificial.orfs1.0490.0141.065
asTX1.4920.0111.524
assignTSSByCage0.0040.0020.005
bamVarName1.8960.0081.930
browseSRA000
codon_usage7.1930.0657.381
codon_usage_exp16.699 0.74917.966
codon_usage_plot7.8380.8289.003
collapse.by.scores0.2400.0270.268
collapse.fastq0.0010.0000.001
collapseDuplicatedReads-GAlignmentPairs-method0.1730.0210.196
collapseDuplicatedReads-GAlignments-method0.0650.0070.073
collapseDuplicatedReads-GRanges-method0.0660.0080.075
collapseDuplicatedReads-data.table-method0.0680.0070.082
collapseDuplicatedReads0.0670.0080.081
combn.pairs1.0590.0181.127
computeFeatures 9.534 0.10910.061
computeFeaturesCage000
config0.0000.0010.000
config.exper0.2380.0170.351
config.save0.0000.0010.001
config_file0.3040.0110.331
convertLibs1.0520.0191.108
convertToOneBasedRanges0.2940.0100.355
convert_bam_to_ofst0.3160.0280.751
convert_to_bigWig2.2930.0572.693
convert_to_covRle1.3560.0311.501
convert_to_covRleList1.8840.0422.162
countOverlapsW0.1590.0020.172
countTable1.0370.0181.126
countTable_regions1.0610.0111.119
covRle0.1090.0030.114
covRleFromGR0.1990.0070.274
covRleList0.0380.0010.044
coverageHeatMap2.6620.0332.920
coveragePerTiling1.6160.0131.717
coverageScorings0.0200.0030.025
create.experiment2.1660.0212.260
defineTrailer0.3680.0030.373
design-experiment-method1.1050.0061.119
detectRibosomeShifts0.0050.0020.007
detect_ribo_orfs15.089 0.85316.101
disengagementScore1.1210.0061.140
distToCds0.5340.0040.541
distToTSS0.4450.0020.456
download.SRA000
download.SRA.metadata0.330.020.76
entropy3.6040.0493.708
experiment-class1.1640.0151.187
export.bed120.0460.0020.048
export.bigWig0.0870.0060.093
export.fstwig0.1300.0060.136
export.ofst-GAlignmentPairs-method0.1440.0040.148
export.ofst-GAlignments-method0.1410.0080.149
export.ofst-GRanges-method0.1450.0060.152
export.ofst0.1390.0020.142
export.wiggle0.0820.0040.086
extendLeaders2.2760.0512.344
extendLeadersUntil3.7790.0173.812
extendTrailers1.4310.0231.470
extendTrailersUntil3.7180.0123.742
extract_run_id0.0010.0010.002
file_ext_without_compression0.0010.0010.001
filepath1.1450.0061.153
filterTranscripts2.9040.0222.933
fimport0.5970.0310.629
findFa0.0050.0010.006
findMapORFs1.1290.0071.138
findORFs0.3570.0070.364
findORFsFasta0.1400.0070.147
findPeaksPerGene2.5360.0222.566
findUORFs0.0020.0010.003
findUORFs_exp11.246 0.06911.639
find_url_ebi0.0570.0100.796
firstEndPerGroup0.1380.0020.140
firstExonPerGroup0.1540.0030.156
firstStartPerGroup0.1420.0020.147
fix_malformed_gff000
flankPerGroup0.2820.0030.288
floss0.6240.0050.633
fpkm0.2910.0020.294
fractionLength0.1490.0010.150
fread.bed0.0670.0160.087
gcContent0.8280.0050.836
geneToSymbol0.0000.0010.001
getGenomeAndAnnotation0.0010.0010.002
get_bioproject_candidates000
get_genome_fasta0.0010.0010.002
get_genome_gtf0.0000.0010.002
get_noncoding_rna0.0000.0010.001
get_phix_genome0.0000.0010.002
get_silva_rRNA0.0000.0000.001