This page was generated on 2020-10-17 11:58:10 -0400 (Sat, 17 Oct 2020).
cellHTS2 2.52.0 Joseph Barry
 
| Snapshot Date: 2020-10-16 14:40:19 -0400 (Fri, 16 Oct 2020) |  
| URL: https://git.bioconductor.org/packages/cellHTS2 |  
| Branch: RELEASE_3_11 |  
| Last Commit: 94333ac |  
| Last Changed Date: 2020-04-27 14:15:21 -0400 (Mon, 27 Apr 2020) |  
 
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R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out"
Copyright (C) 2020 The R Foundation for Statistical Computing
Platform: x86_64-apple-darwin17.0 (64-bit)
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> ## cat tests/test.R | R --vanilla
> ## cellHTS2 crash test on various conditions
> library(cellHTS2)
Loading required package: RColorBrewer
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
    IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which, which.max, which.min
Welcome to Bioconductor
    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: genefilter
Loading required package: splots
Loading required package: vsn
Loading required package: hwriter
Loading required package: locfit
locfit 1.5-9.4 	 2020-03-24
Loading required package: grid
> path <- system.file("testscreen", package="cellHTS2")
> 
> testPlatelist=function(platelist, normalize=TRUE)
+ {
+     x <- readPlateList(platelist, name="test", path=path)
+     x <- configure(x, descripFile="description.txt", confFile="plateconf.txt",
+                    logFile="screenlog.txt", path=path)
+     
+     if (normalize)
+     {
+         ## normalize results
+         xn <- normalizePlates(x, scale="multiplicative", log=FALSE, method="median",
+                               varianceAdjust="none")
+         
+         ## score and summarize replicates
+         xsc <- scoreReplicates(xn, sign="-", method="zscore")
+         xsc <- summarizeReplicates(xsc, summary="mean")
+     }
+     
+     ## write reports
+     outdir <- file.path(tempdir(),platelist,'raw')
+     mainScriptFile <-  system.file("scripts/dummy.R", package="cellHTS2")
+     writeReport(raw=x, force=TRUE, plotPlateArgs = TRUE,imageScreenArgs = list(zrange=c( -4, 8), ar=1),
+                 map=TRUE, outdir=outdir, mainScriptFile=mainScriptFile)
+     if (interactive()) browseURL(file.path(outdir,'index.html'))
+     if (normalize)
+     {
+         outdir <- file.path(tempdir(),platelist,'norm')
+         writeReport(raw=x, normalized=xn, scored=xsc, force=TRUE, plotPlateArgs = TRUE,
+                     imageScreenArgs = list(zrange=c( -4, 8), ar=1), map=TRUE, outdir=outdir,
+                     mainScriptFile=mainScriptFile)
+         if (interactive()) browseURL(file.path(outdir,'index.html'))
+     }
+ }
> 
> ######
> ## 2 plates, 2 replicates, 1 channel
> testPlatelist('platelist221.txt')
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 6)
33% done (step 2 of 6)
38% done (step 3 of 6)
42% done (step 3 of 6)
52% done (step 4 of 6)
88% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 6 of 6)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist221.txt/raw/index.html
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw, normalized and scored data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 8)
17% done (step 2 of 8)
19% done (step 3 of 8)
22% done (step 3 of 8)
27% done (step 4 of 8)
46% done (step 5 of 8)
92% done (step 6 of 8)
94% done (step 7 of 8)
100% done (step 8 of 8)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist221.txt/norm/index.html
Warning messages:
1: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
2: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
3: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
4: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
> 
> ######
> ## 2 plates, 1 replicate, 2 channels
> testPlatelist('platelist212.txt', normalize=FALSE)
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 6)
33% done (step 2 of 6)
38% done (step 3 of 6)
42% done (step 3 of 6)
52% done (step 4 of 6)
88% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 6 of 6)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist212.txt/raw/index.html
Warning messages:
1: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
2: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
> 
> ######
> ## 2 plates, 1 replicate, 3 channels
> testPlatelist('platelist213.txt', normalize=FALSE)
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 6)
26% done (step 2 of 6)
31% done (step 3 of 6)
37% done (step 3 of 6)
48% done (step 4 of 6)
91% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 6 of 6)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist213.txt/raw/index.html
Warning messages:
1: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
2: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
> 
> ######
> ## 2 plates, 2 replicates, 2 channels
> testPlatelist('platelist222.txt', normalize=FALSE)
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 6)
21% done (step 2 of 6)
27% done (step 3 of 6)
33% done (step 3 of 6)
46% done (step 4 of 6)
92% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 6 of 6)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist222.txt/raw/index.html
Warning messages:
1: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
2: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
> 
> ######
> ## 2 plates, 1 replicates, 1 channel
> testPlatelist('platelist211.txt')
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 6)
46% done (step 2 of 6)
49% done (step 3 of 6)
52% done (step 3 of 6)
59% done (step 4 of 6)
84% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 6 of 6)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist211.txt/raw/index.html
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw, normalized and scored data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 8)
28% done (step 2 of 8)
30% done (step 3 of 8)
31% done (step 3 of 8)
36% done (step 4 of 8)
51% done (step 5 of 8)
88% done (step 6 of 8)
90% done (step 7 of 8)
100% done (step 8 of 8)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist211.txt/norm/index.html
Warning messages:
1: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
2: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
3: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
4: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
> 
> ######
> ## 2 plates, 3 replicates, 3 channels
> testPlatelist('platelist233.txt', normalize=FALSE)
cellHTS2 is busy creating HTML pages for 'test'. 
Found raw data.
State:
configured=TRUE, annotated=FALSE
0% done (step 1 of 6)
11% done (step 2 of 6)
18% done (step 3 of 6)
25% done (step 3 of 6)
40% done (step 4 of 6)
96% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 5 of 6)
100% done (step 6 of 6)
Report was successfully generated in folder /tmp/RtmpJC6QBe/platelist233.txt/raw/index.html
Warning messages:
1: The plotPlateArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
2: The imageScreenArgs argument is deprecated. Please use the settings infrastructure as explained in '?setSettings'. 
> 
> proc.time()
   user  system elapsed 
 45.341   2.265  50.236