Package: annotation Version: 1.33.0 Depends: R (>= 3.3.0), VariantAnnotation, AnnotationHub, Organism.dplyr, TxDb.Hsapiens.UCSC.hg19.knownGene, TxDb.Hsapiens.UCSC.hg38.knownGene, TxDb.Mmusculus.UCSC.mm10.ensGene, org.Hs.eg.db, org.Mm.eg.db, Homo.sapiens, BSgenome.Hsapiens.UCSC.hg19, biomaRt, BSgenome, TxDb.Athaliana.BioMart.plantsmart22 Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 24273a2a3349e82733efc007aa6cbd1a NeedsCompilation: no Title: Genomic Annotation Resources Description: Annotation resources make up a significant proportion of the Bioconductor project. And there are also a diverse set of online resources available which are accessed using specific packages. This walkthrough will describe the most popular of these resources and give some high level examples on how to use them. biocViews: AnnotationWorkflow, Workflow Author: Marc RJ Carlson [aut], Herve Pages [aut], Sonali Arora [aut], Valerie Obenchain [aut], Martin Morgan [aut], Lori Shepherd [ctb], Bioconductor Package Maintainer [cre] Maintainer: Bioconductor Package Maintainer URL: http://bioconductor.org/packages/annotation VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/annotation git_branch: devel git_last_commit: 12ea099 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/annotation_1.33.0.tar.gz vignettes: vignettes/annotation/inst/doc/Annotating_Genomic_Ranges.html, vignettes/annotation/inst/doc/Annotation_Resources.html vignetteTitles: Annotating Genomic Ranges, Genomic Annotation Resources hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/annotation/inst/doc/Annotating_Genomic_Ranges.R, vignettes/annotation/inst/doc/Annotation_Resources.R dependencyCount: 118 Package: arrays Version: 1.35.0 Depends: R (>= 3.0.0) Suggests: affy, limma, hgfocuscdf, knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 3b6c684dd08357b11507bd647687acf6 NeedsCompilation: no Title: Using Bioconductor for Microarray Analysis Description: Using Bioconductor for Microarray Analysis workflow biocViews: Workflow, BasicWorkflow Author: Bioconductor Package Maintainer [aut, cre] Maintainer: Bioconductor Package Maintainer VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/arrays git_branch: devel git_last_commit: a25feab git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/arrays_1.35.0.tar.gz vignettes: vignettes/arrays/inst/doc/arrays.html vignetteTitles: Using Bioconductor for Microarray Analysis hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/arrays/inst/doc/arrays.R dependencyCount: 0 Package: BP4RNAseq Version: 1.19.0 Depends: R (>= 4.0.0) Imports: dplyr, fastqcr, stringr, tidyr, stats, utils, magrittr, reticulate Suggests: knitr, rmarkdown, testthat License: GPL-2 MD5sum: c753ec93ff44954737dc38d2377d3477 NeedsCompilation: no Title: A babysitter's package for reproducible RNA-seq analysis Description: An automated pipe for reproducible RNA-seq analysis with the minimal efforts from researchers. The package can process bulk RNA-seq data and single-cell RNA-seq data. You can only provide the taxa name and the accession id of RNA-seq data deposited in the National Center for Biotechnology Information (NCBI). After a cup of tea or longer, you will get formated gene expression data as gene count and transcript count based on both alignment-based and alignment-free workflows. biocViews: GeneExpressionWorkflow Author: Shanwen Sun [cre, aut], Lei Xu [aut], Quan Zou [aut] Maintainer: Shanwen Sun SystemRequirements: UNIX, SRA Toolkit=2.10.3, Entrez Direct=13.3, FastQC=v0.11.9, Cutadapt=2.10, datasets, SAMtools=1.9, HISAT2=2.2.0, StringTie=2.1.1, Salmon=1.2.1 VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/BP4RNAseq git_branch: devel git_last_commit: 1b92150 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/BP4RNAseq_1.19.0.tar.gz vignettes: vignettes/BP4RNAseq/inst/doc/vignette.html vignetteTitles: BP4RNAseq vignette hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/BP4RNAseq/inst/doc/vignette.R dependencyCount: 88 Package: CAGEWorkflow Version: 1.25.0 Depends: R (>= 3.6.0), CAGEfightR, nanotubes Suggests: knitr, magick, rmarkdown, BiocStyle, BiocWorkflowTools, pheatmap, ggseqlogo, viridis, magrittr, ggforce, ggthemes, tidyverse, dplyr, GenomicRanges, SummarizedExperiment, GenomicFeatures, BiocParallel, InteractionSet, Gviz, DESeq2, limma, edgeR, statmod, BiasedUrn, sva, TFBSTools, motifmatchr, pathview, BSgenome.Mmusculus.UCSC.mm9, TxDb.Mmusculus.UCSC.mm9.knownGene, org.Mm.eg.db, JASPAR2016, png License: GPL-3 MD5sum: ab1b53f473a4b8d5dadadeb266c9df6e NeedsCompilation: no Title: A step-by-step guide to analyzing CAGE data using R/Bioconductor Description: Workflow for analyzing Cap Analysis of Gene Expression (CAGE) data using R/Bioconductor. biocViews: GeneExpressionWorkflow, AnnotationWorkflow Author: Malte Thodberg [aut, cre] Maintainer: Malte Thodberg VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/CAGEWorkflow git_branch: devel git_last_commit: 82d84bf git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/CAGEWorkflow_1.25.0.tar.gz vignettes: vignettes/CAGEWorkflow/inst/doc/CAGEWorkflow.html vignetteTitles: CAGEWorkflow hasREADME: FALSE hasNEWS: TRUE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/CAGEWorkflow/inst/doc/CAGEWorkflow.R dependencyCount: 162 Package: chipseqDB Version: 1.33.0 Suggests: chipseqDBData, BiocStyle, BiocFileCache, ChIPpeakAnno, Gviz, Rsamtools, TxDb.Mmusculus.UCSC.mm10.knownGene, csaw, edgeR, knitr, org.Mm.eg.db, rtracklayer, rmarkdown License: Artistic-2.0 MD5sum: a6f7c60b7ed8108d694e2754c771d0b2 NeedsCompilation: no Title: A Bioconductor Workflow to Detect Differential Binding in ChIP-seq Data Description: Describes a computational workflow for performing a DB analysis with sliding windows. The aim is to facilitate the practical implementation of window-based DB analyses by providing detailed code and expected output. The workflow described here applies to any ChIP-seq experiment with multiple experimental conditions and multiple biological samples in one or more of the conditions. It detects and summarizes DB regions between conditions in a de novo manner, i.e., without making any prior assumptions about the location or width of bound regions. Detected regions are then annotated according to their proximity to genes. biocViews: ImmunoOncologyWorkflow, Workflow, EpigeneticsWorkflow Author: Aaron Lun [aut, cre], Gordon Smyth [aut] Maintainer: Aaron Lun URL: https://www.bioconductor.org/help/workflows/chipseqDB/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/chipseqDB git_branch: devel git_last_commit: bc12f94 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/chipseqDB_1.33.0.tar.gz vignettes: vignettes/chipseqDB/inst/doc/cbp.html, vignettes/chipseqDB/inst/doc/h3k27me3.html, vignettes/chipseqDB/inst/doc/h3k9ac.html, vignettes/chipseqDB/inst/doc/intro.html vignetteTitles: 3. Differential binding of CBP in fibroblasts, 4. Differential enrichment of H3K27me3 in lung epithelium, 2. Differential enrichment of H3K9ac in B cells, 1. Introduction hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/chipseqDB/inst/doc/cbp.R dependencyCount: 0 Package: csawUsersGuide Version: 1.25.0 Suggests: knitr, BiocStyle, BiocManager License: GPL-3 MD5sum: 88bea359d4b096cd24816bdd77077227 NeedsCompilation: no Title: csaw User's Guide Description: A user's guide for the csaw package for detecting differentially bound regions in ChIP-seq data. Describes how to read in BAM files to obtain a per-window count matrix, filtering to obtain high-abundance windows of interest, normalization of sample-specific biases, testing for differential binding, consolidation of per-window results to obtain per-region statistics, and annotation and visualization of the DB results. biocViews: Workflow, EpigeneticsWorkflow Author: Aaron Lun [aut, cre] Maintainer: Aaron Lun VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/csawUsersGuide git_branch: devel git_last_commit: d2a08b2 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/csawUsersGuide_1.25.0.tar.gz vignettes: vignettes/csawUsersGuide/inst/doc/csaw.pdf vignetteTitles: User's guide hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/csawUsersGuide/inst/doc/csaw.R dependencyCount: 0 Package: cytofWorkflow Version: 1.33.0 Depends: R (>= 3.6.0), BiocStyle, knitr, readxl, CATALYST, diffcyt, HDCytoData, uwot, cowplot Suggests: knitcitations, markdown, rmarkdown License: Artistic-2.0 MD5sum: 54dac6bb7c8f602a279ed92b5d63269d NeedsCompilation: no Title: CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets Description: High-dimensional mass and flow cytometry (HDCyto) experiments have become a method of choice for high-throughput interrogation and characterization of cell populations. Here, we present an updated R-based pipeline for differential analyses of HDCyto data, largely based on Bioconductor packages. We computationally define cell populations using FlowSOM clustering, and facilitate an optional but reproducible strategy for manual merging of algorithm-generated clusters. Our workflow offers different analysis paths, including association of cell type abundance with a phenotype or changes in signaling markers within specific subpopulations, or differential analyses of aggregated signals. Importantly, the differential analyses we show are based on regression frameworks where the HDCyto data is the response; thus, we are able to model arbitrary experimental designs, such as those with batch effects, paired designs and so on. In particular, we apply generalized linear mixed models or linear mixed models to analyses of cell population abundance or cell-population-specific analyses of signaling markers, allowing overdispersion in cell count or aggregated signals across samples to be appropriately modeled. To support the formal statistical analyses, we encourage exploratory data analysis at every step, including quality control (e.g., multi-dimensional scaling plots), reporting of clustering results (dimensionality reduction, heatmaps with dendrograms) and differential analyses (e.g., plots of aggregated signals). biocViews: ImmunoOncologyWorkflow, Workflow, SingleCellWorkflow Author: Malgorzata Nowicka [aut], Helena L. Crowell [aut], Mark D. Robinson [aut, cre] Maintainer: Mark D. Robinson URL: https://github.com/markrobinsonuzh/cytofWorkflow VignetteBuilder: knitr BugReports: https://github.com/markrobinsonuzh/cytofWorkflow/issues git_url: https://git.bioconductor.org/packages/cytofWorkflow git_branch: devel git_last_commit: a94e07c git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/cytofWorkflow_1.33.0.tar.gz vignettes: vignettes/cytofWorkflow/inst/doc/cytofWorkflow.html vignetteTitles: A workflow for differential discovery in high-throughput high-dimensional cytometry datasets hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE dependencyCount: 228 Package: ExpressionNormalizationWorkflow Version: 1.35.0 Imports: Biobase (>= 2.24.0), limma (>= 3.20.9), lme4 (>= 1.1.7), matrixStats (>= 0.10.3), pvca (>= 1.4.0), snm (>= 1.12.0), sva (>= 3.10.0), vsn (>= 3.32.0) Suggests: knitr, BiocStyle License: GPL (>=3) MD5sum: 0d204e62ceb4b3b217c26dfb91c8c968 NeedsCompilation: no Title: Gene Expression Normalization Workflow Description: An extensive, customized expression normalization workflow incorporating Supervised Normalization of Microarryas(SNM), Surrogate Variable Analysis(SVA) and Principal Variance Component Analysis to identify batch effects and remove them from the expression data to enhance the ability to detect the underlying biological signals. biocViews: ImmunoOncologyWorkflow, Workflow, GeneExpressionWorkflow Author: Karthikeyan Murugesan [aut, cre], Greg Gibson [sad, ths] Maintainer: Karthikeyan Murugesan VignetteBuilder: knitr BugReports: https://github.com/ git_url: https://git.bioconductor.org/packages/ExpressionNormalizationWorkflow git_branch: devel git_last_commit: 43e01e3 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/ExpressionNormalizationWorkflow_1.35.0.tar.gz vignettes: vignettes/ExpressionNormalizationWorkflow/inst/doc/genExpNrm.html vignetteTitles: Gene Expression Normalization Workflow hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/ExpressionNormalizationWorkflow/inst/doc/genExpNrm.R dependencyCount: 102 Package: fluentGenomics Version: 1.21.0 Depends: R (>= 4.0) Imports: plyranges (>= 1.7.7), dplyr, SummarizedExperiment, readr, stats, utils Suggests: knitr, rmarkdown, bookdown, rappdirs, BiocFileCache, DESeq2, limma, ggplot2, tidyr, tximeta (>= 1.4.2), macrophage (>= 1.2.0), License: MIT + file LICENSE MD5sum: 7b022c97b7da1aeb75b0debf3ea26002 NeedsCompilation: no Title: A plyranges and tximeta workflow Description: An extended workflow using the plyranges and tximeta packages for fluent genomic data analysis. Use tximeta to correctly import RNA-seq transcript quantifications and summarize them to gene counts for downstream analysis. Use plyranges for clearly expressing operations over genomic coordinates and to combine results from differential expression and differential accessibility analyses. biocViews: Workflow, BasicWorkflow, GeneExpressionWorkflow Author: Stuart Lee [aut, cre] (ORCID: ), Michael Love [aut, ctb] Maintainer: Stuart Lee URL: https://github.com/sa-lee/fluentGenomics VignetteBuilder: knitr, rmarkdown BugReports: https://github.com/sa-lee/fluentGenomics/issues git_url: https://git.bioconductor.org/packages/fluentGenomics git_branch: devel git_last_commit: b942cf1 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/fluentGenomics_1.21.0.tar.gz vignettes: vignettes/fluentGenomics/inst/doc/fluentGenomics.html vignetteTitles: fluentGenomics hasREADME: FALSE hasNEWS: TRUE hasINSTALL: FALSE hasLICENSE: TRUE Rfiles: vignettes/fluentGenomics/inst/doc/fluentGenomics.R dependencyCount: 80 Package: generegulation Version: 1.33.0 Depends: R (>= 3.3.0), BSgenome.Scerevisiae.UCSC.sacCer3, Biostrings, GenomicFeatures, MotifDb, S4Vectors, TxDb.Scerevisiae.UCSC.sacCer3.sgdGene, motifStack, org.Sc.sgd.db, seqLogo Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 0232037b414d6a9868cecc0a25ac9363 NeedsCompilation: no Title: Finding Candidate Binding Sites for Known Transcription Factors via Sequence Matching Description: The binding of transcription factor proteins (TFs) to DNA promoter regions upstream of gene transcription start sites (TSSs) is one of the most important mechanisms by which gene expression, and thus many cellular processes, are controlled. Though in recent years many new kinds of data have become available for identifying transcription factor binding sites (TFBSs) -- ChIP-seq and DNase I hypersensitivity regions among them -- sequence matching continues to play an important role. In this workflow we demonstrate Bioconductor techniques for finding candidate TF binding sites in DNA sequence using the model organism Saccharomyces cerevisiae. The methods demonstrated here apply equally well to other organisms. biocViews: Workflow, EpigeneticsWorkflow Author: Bioconductor Package Maintainer [aut, cre] Maintainer: Bioconductor Package Maintainer URL: https://www.bioconductor.org/help/workflows/generegulation/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/generegulation git_branch: devel git_last_commit: 63557a6 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/generegulation_1.33.0.tar.gz vignettes: vignettes/generegulation/inst/doc/generegulation.html vignetteTitles: Finding Candidate Binding Sites for Known Transcription Factors via Sequence Matching hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/generegulation/inst/doc/generegulation.R dependencyCount: 129 Package: highthroughputassays Version: 1.33.0 Depends: R (>= 3.3.0), flowCore, flowStats, flowWorkspace Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 5a23a24f70f3b647e81634b521ffd5fe NeedsCompilation: no Title: Using Bioconductor with High Throughput Assays Description: The workflow illustrates use of the flow cytometry packages to load, transform and visualize the flow data and gate certain populations in the dataset. The workflow loads the `flowCore`, `flowStats` and `flowWorkspace` packages and its dependencies. It loads the ITN data with 15 samples, each of which includes, in addition to FSC and SSC, 5 fluorescence channels: CD3, CD4, CD8, CD69 and HLADR. biocViews: ImmunoOncologyWorkflow, Workflow, ProteomicsWorkflow Author: Bioconductor Package Maintainer [aut, cre] Maintainer: Bioconductor Package Maintainer URL: https://www.bioconductor.org/help/workflows/highthroughputassays/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/highthroughputassays git_branch: devel git_last_commit: 78ca485 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/highthroughputassays_1.33.0.tar.gz vignettes: vignettes/highthroughputassays/inst/doc/high-throughput-assays.html vignetteTitles: Using Bioconductor with High Throughput Assays hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/highthroughputassays/inst/doc/high-throughput-assays.R dependencyCount: 99 Package: liftOver Version: 1.33.0 Depends: R (>= 3.3.0), gwascat, GenomicRanges, rtracklayer, Homo.sapiens, BiocGenerics Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: bc72b60f4df5fe23391b64cd74b3aaeb NeedsCompilation: no Title: Changing genomic coordinate systems with rtracklayer::liftOver Description: The liftOver facilities developed in conjunction with the UCSC browser track infrastructure are available for transforming data in GRanges formats. This is illustrated here with an image of the EBI/NHGRI GWAS catalog that is, as of May 10 2017, distributed with coordinates defined by NCBI build hg38. biocViews: Workflow, BasicWorkflow Author: Bioconductor Package Maintainer [aut, cre] Maintainer: Bioconductor Package Maintainer URL: https://www.bioconductor.org/help/workflows/liftOver/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/liftOver git_branch: devel git_last_commit: b7069ac git_last_commit_date: 2025-04-15 Date/Publication: 2025-05-02 source.ver: src/contrib/liftOver_1.33.0.tar.gz vignettes: vignettes/liftOver/inst/doc/liftov.html vignetteTitles: Changing genomic coordinate systems with rtracklayer::liftOver hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/liftOver/inst/doc/liftov.R suggestsMe: bedbaser dependencyCount: 116 Package: methylationArrayAnalysis Version: 1.33.1 Depends: R (>= 3.3.0), knitr, rmarkdown, BiocStyle, limma, minfi, IlluminaHumanMethylation450kanno.ilmn12.hg19, IlluminaHumanMethylation450kmanifest, RColorBrewer, missMethyl, matrixStats, minfiData, Gviz, DMRcate, stringr, FlowSorted.Blood.450k License: Artistic-2.0 MD5sum: f295af5775b4d175b4cf5ff923a652bb NeedsCompilation: no Title: A cross-package Bioconductor workflow for analysing methylation array data Description: Methylation in the human genome is known to be associated with development and disease. The Illumina Infinium methylation arrays are by far the most common way to interrogate methylation across the human genome. This Bioconductor workflow uses multiple packages for the analysis of methylation array data. Specifically, we demonstrate the steps involved in a typical differential methylation analysis pipeline including: quality control, filtering, normalization, data exploration and statistical testing for probe-wise differential methylation. We further outline other analyses such as differential methylation of regions, differential variability analysis, estimating cell type composition and gene ontology testing. Finally, we provide some examples of how to visualise methylation array data. biocViews: Workflow, EpigeneticsWorkflow Author: Jovana Maksimovic [aut, cre] Maintainer: Jovana Maksimovic VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/methylationArrayAnalysis git_branch: devel git_last_commit: 1bfdffc git_last_commit_date: 2025-05-29 Date/Publication: 2025-05-30 source.ver: src/contrib/methylationArrayAnalysis_1.33.1.tar.gz vignettes: vignettes/methylationArrayAnalysis/inst/doc/methylationArrayAnalysis.html vignetteTitles: A cross-package Bioconductor workflow for analysing methylation array data hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/methylationArrayAnalysis/inst/doc/methylationArrayAnalysis.R dependencyCount: 226 Package: recountWorkflow Version: 1.33.0 Depends: R (>= 3.6.0) Imports: recount, GenomicRanges, limma, edgeR, DESeq2, pheatmap, regionReport, clusterProfiler, org.Hs.eg.db, gplots, derfinder, GenomicState, bumphunter, derfinderPlot Suggests: BiocStyle, BiocWorkflowTools, knitr, magick, sessioninfo, rmarkdown License: Artistic-2.0 MD5sum: 1595bb259a26d3cb55cf1fa7690c34eb NeedsCompilation: no Title: recount workflow: accessing over 70,000 human RNA-seq samples with Bioconductor Description: The recount2 resource is composed of over 70,000 uniformly processed human RNA-seq samples spanning TCGA and SRA, including GTEx. The processed data can be accessed via the recount2 website and the recount Bioconductor package. This workflow explains in detail how to use the recount package and how to integrate it with other Bioconductor packages for several analyses that can be carried out with the recount2 resource. In particular, we describe how the coverage count matrices were computed in recount2 as well as different ways of obtaining public metadata, which can facilitate downstream analyses. Step-by-step directions show how to do a gene level differential expression analysis, visualize base-level genome coverage data, and perform an analyses at multiple feature levels. This workflow thus provides further information to understand the data in recount2 and a compendium of R code to use the data. biocViews: Workflow, ResourceQueryingWorkflow Author: Leonardo Collado-Torres [aut, cre], Abhinav Nellore [ctb], Andrew E. Jaffe [ctb] Maintainer: Leonardo Collado-Torres URL: https://github.com/LieberInstitute/recountWorkflow VignetteBuilder: knitr BugReports: https://support.bioconductor.org/t/recountWorkflow/ git_url: https://git.bioconductor.org/packages/recountWorkflow git_branch: devel git_last_commit: 4f05efe git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/recountWorkflow_1.33.0.tar.gz vignettes: vignettes/recountWorkflow/inst/doc/recount-workflow.html vignetteTitles: recount workflow: accessing over 70,,000 human RNA-seq samples with Bioconductor hasREADME: FALSE hasNEWS: TRUE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/recountWorkflow/inst/doc/recount-workflow.R dependencyCount: 237 Package: RNAseq123 Version: 1.33.0 Depends: R (>= 3.3.0), Glimma (>= 1.1.9), limma, edgeR, gplots, RColorBrewer, Mus.musculus, R.utils, TeachingDemos, statmod, BiocWorkflowTools Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 7622d6f77cb355ae229a54c93fb923a8 NeedsCompilation: no Title: RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR Description: R package that supports the F1000Research workflow article on RNA-seq analysis using limma, Glimma and edgeR by Law et al. (2016). biocViews: Workflow, GeneExpressionWorkflow, ImmunoOncologyWorkflow Author: Charity Law, Monther Alhamdoosh, Shian Su, Xueyi Dong, Luyi Tian, Gordon Smyth and Matthew Ritchie Maintainer: Matthew Ritchie URL: https://f1000research.com/articles/5-1408/v3 VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/RNAseq123 git_branch: devel git_last_commit: 138b4d1 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/RNAseq123_1.33.0.tar.gz vignettes: vignettes/RNAseq123/inst/doc/designmatrices.html, vignettes/RNAseq123/inst/doc/limmaWorkflow_CHN.html, vignettes/RNAseq123/inst/doc/limmaWorkflow.html vignetteTitles: A guide to creating design matrices for gene expression experiments (English version), RNA-seq analysis is easy as 1-2-3 with limma,, Glimma and edgeR (Chinese version), RNA-seq analysis is easy as 1-2-3 with limma,, Glimma and edgeR (English version) hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/RNAseq123/inst/doc/designmatrices.R, vignettes/RNAseq123/inst/doc/limmaWorkflow_CHN.R, vignettes/RNAseq123/inst/doc/limmaWorkflow.R dependencyCount: 153 Package: rnaseqDTU Version: 1.29.0 Depends: R (>= 3.5.0), DRIMSeq, DEXSeq, stageR, DESeq2, edgeR, rafalib, devtools Suggests: knitr, rmarkdown License: Artistic-2.0 MD5sum: 0ecc32f3bb3c47c325f274302229687b NeedsCompilation: no Title: RNA-seq workflow for differential transcript usage following Salmon quantification Description: RNA-seq workflow for differential transcript usage (DTU) following Salmon quantification. This workflow uses Bioconductor packages tximport, DRIMSeq, and DEXSeq to perform a DTU analysis on simulated data. It also shows how to use stageR to perform two-stage testing of DTU, a statistical framework to screen at the gene level and then confirm which transcripts within the significant genes show evidence of DTU. biocViews: Workflow, GeneExpressionWorkflow, ImmunoOncologyWorkflow Author: Michael Love [aut, cre], Charlotte Soneson [aut], Rob Patro [aut] Maintainer: Michael Love URL: https://github.com/thelovelab/rnaseqDTU/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/rnaseqDTU git_branch: devel git_last_commit: 6891650 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/rnaseqDTU_1.29.0.tar.gz vignettes: vignettes/rnaseqDTU/inst/doc/rnaseqDTU.html vignetteTitles: RNA-seq workflow for differential transcript usage following Salmon quantification hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/rnaseqDTU/inst/doc/rnaseqDTU.R dependencyCount: 183 Package: rnaseqGene Version: 1.33.0 Depends: R (>= 3.3.0), BiocStyle, airway (>= 1.5.3), tximeta, magrittr, DESeq2, apeglm, vsn, dplyr, ggplot2, hexbin, pheatmap, RColorBrewer, PoiClaClu, glmpca, ggbeeswarm, genefilter, AnnotationDbi, org.Hs.eg.db, Gviz, sva, RUVSeq, fission Suggests: knitr, rmarkdown License: Artistic-2.0 MD5sum: af8599771f250055d46a798c822ad2ce NeedsCompilation: no Title: RNA-seq workflow: gene-level exploratory analysis and differential expression Description: Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were aligned to the reference genome, and prepare a count matrix which tallies the number of RNA-seq reads/fragments within each gene for each sample. We will perform exploratory data analysis (EDA) for quality assessment and to explore the relationship between samples, perform differential gene expression analysis, and visually explore the results. biocViews: Workflow, GeneExpressionWorkflow, ImmunoOncologyWorkflow Author: Michael Love [aut, cre] Maintainer: Michael Love URL: https://github.com/thelovelab/rnaseqGene/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/rnaseqGene git_branch: devel git_last_commit: b1a0320 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/rnaseqGene_1.33.0.tar.gz vignettes: vignettes/rnaseqGene/inst/doc/rnaseqGene.html vignetteTitles: RNA-seq workflow at the gene level hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/rnaseqGene/inst/doc/rnaseqGene.R dependencyCount: 204 Package: RnaSeqGeneEdgeRQL Version: 1.33.0 Depends: R (>= 3.3.0), edgeR (>= 4.3.6), gplots, org.Mm.eg.db, GO.db, BiocStyle Suggests: knitr, knitcitations, rmarkdown License: Artistic-2.0 MD5sum: d1fa3a44f61c6d9253a8d39ff3a67f22 NeedsCompilation: no Title: Gene-level RNA-seq differential expression and pathway analysis using Rsubread and the edgeR quasi-likelihood pipeline Description: This workflow package provides, through its vignette, a complete case study analysis of an RNA-Seq experiment using the Rsubread and edgeR packages. The workflow starts from read alignment and continues on to data exploration, to differential expression and, finally, to pathway analysis. The analysis includes publication quality plots, GO and KEGG analyses, and the analysis of a expression signature as generated by a prior experiment. biocViews: Workflow, GeneExpressionWorkflow, ImmunoOncologyWorkflow Author: Yunshun Chen, Aaron Lun, Gordon Smyth Maintainer: Yunshun Chen URL: http://f1000research.com/articles/5-1438/v2 VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/RnaSeqGeneEdgeRQL git_branch: devel git_last_commit: 4e6962b git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/RnaSeqGeneEdgeRQL_1.33.0.tar.gz vignettes: vignettes/RnaSeqGeneEdgeRQL/inst/doc/edgeRQL.html vignetteTitles: From reads to genes to pathways: differential expression analysis of RNA-Seq experiments using Rsubread and the edgeR quasi-likelihood pipeline hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/RnaSeqGeneEdgeRQL/inst/doc/edgeRQL.R dependencyCount: 76 Package: sequencing Version: 1.33.0 Depends: R (>= 3.3.0), GenomicRanges, GenomicAlignments, Biostrings, Rsamtools, ShortRead, BiocParallel, rtracklayer, VariantAnnotation, AnnotationHub, BSgenome.Hsapiens.UCSC.hg19, RNAseqData.HNRNPC.bam.chr14 Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 17fcd0baa44417d91ed8e7c97df2b666 NeedsCompilation: no Title: Introduction to Bioconductor for Sequence Data Description: Bioconductor enables the analysis and comprehension of high- throughput genomic data. We have a vast number of packages that allow rigorous statistical analysis of large data while keeping technological artifacts in mind. Bioconductor helps users place their analytic results into biological context, with rich opportunities for visualization. Reproducibility is an important goal in Bioconductor analyses. Different types of analysis can be carried out using Bioconductor, for example; Sequencing : RNASeq, ChIPSeq, variants, copy number etc.; Microarrays: expression, SNP, etc.; Domain specific analysis : Flow cytometry, Proteomics etc. For these analyses, one typically imports and works with diverse sequence-related file types, including fasta, fastq, BAM, gtf, bed, and wig files, among others. Bioconductor packages support import, common and advanced sequence manipulation operations such as trimming, transformation, and alignment including quality assessment. biocViews: ImmunoOncologyWorkflow, Workflow, BasicWorkflow Author: Sonali Arora [aut], Martin Morgan [aut], Bioconductor Package Maintainer [cre] Maintainer: Bioconductor Package Maintainer URL: https://www.bioconductor.org/help/workflows/sequencing/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/sequencing git_branch: devel git_last_commit: 5061618 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/sequencing_1.33.0.tar.gz vignettes: vignettes/sequencing/inst/doc/sequencing.html vignetteTitles: Introduction to Bioconductor for Sequence Data hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/sequencing/inst/doc/sequencing.R dependencyCount: 110 Package: simpleSingleCell Version: 1.33.0 Imports: utils, methods, knitr, callr, rmarkdown, CodeDepends, BiocStyle Suggests: readxl, R.utils, SingleCellExperiment, scater, scran, limma, BiocFileCache, org.Mm.eg.db License: Artistic-2.0 MD5sum: 940c12a9d546a2a20eb839a61d6492a4 NeedsCompilation: no Title: A step-by-step workflow for low-level analysis of single-cell RNA-seq data with Bioconductor Description: Once a proud workflow package, this is now a shell of its former self. Almost all of its content has been cannibalized for use in the "Orchestrating Single-Cell Analyses with Bioconductor" book at https://osca.bioconductor.org. Most vignettes here are retained as reminders of the glory that once was, also providing redirection for existing external links to the relevant OSCA book chapters. biocViews: ImmunoOncologyWorkflow, Workflow, SingleCellWorkflow Author: Aaron Lun [aut, cre], Davis McCarthy [aut], John Marioni [aut] Maintainer: Aaron Lun URL: https://www.bioconductor.org/help/workflows/simpleSingleCell/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/simpleSingleCell git_branch: devel git_last_commit: b931cf9 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/simpleSingleCell_1.33.0.tar.gz vignettes: vignettes/simpleSingleCell/inst/doc/batch.html, vignettes/simpleSingleCell/inst/doc/bigdata.html, vignettes/simpleSingleCell/inst/doc/de.html, vignettes/simpleSingleCell/inst/doc/doublets.html, vignettes/simpleSingleCell/inst/doc/intro.html, vignettes/simpleSingleCell/inst/doc/misc.html, vignettes/simpleSingleCell/inst/doc/multibatch.html, vignettes/simpleSingleCell/inst/doc/qc.html, vignettes/simpleSingleCell/inst/doc/reads.html, vignettes/simpleSingleCell/inst/doc/spike.html, vignettes/simpleSingleCell/inst/doc/tenx.html, vignettes/simpleSingleCell/inst/doc/umis.html, vignettes/simpleSingleCell/inst/doc/var.html vignetteTitles: 05. Correcting batch effects, 12. Scalability for big data, 10. Detecting differential expression, 08. Detecting doublets, 01. Introduction, 13. Further analysis strategies, 11. Advanced batch correction, 06. Quality control details, 02. Read count data, 07. Spike-in normalization, 04. Droplet-based data, 03. UMI count data, 09. Advanced variance modelling hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/simpleSingleCell/inst/doc/misc.R dependencyCount: 45 Package: spicyWorkflow Version: 1.9.0 Depends: R (>= 4.3.0) Suggests: knitr, rmarkdown, BiocStyle, EBImage, cytomapper, ggplot2, ggpubr, lisaClust, spicyR, ClassifyR, scater, dplyr, simpleSeg, FuseSOM, HDF5Array, parallel, tidySingleCellExperiment, SpatialDatasets, Statial, treekoR License: GPL-3 MD5sum: 22841760cee41e0ce8e539720069c982 NeedsCompilation: no Title: Performing a Spatial Analysis of Multiplexed Tissue Imaging Data Description: We have developed an analytical framework for analysing data from high dimensional in situ cytometry assays including CODEX, CycIF, IMC and High Definition Spatial Transcriptomics. This framework makes use of functionality from our Bioconductor packages spicyR, lisaClust, scFeatures, FuseSOM, simpleSeg and ClassifyR and contains most of the key steps which are needed to interrogate the comprehensive spatial information generated by these exciting new technologies including cell segmentation, feature normalisation, cell type identification, micro-environment characterisation, spatial hypothesis testing and patient classification. Ultimately, our modular analysis framework provides a cohesive and accessible entry point into spatially resolved single cell data analysis for any R-based bioinformatician. biocViews: Workflow, SpatialWorkflow, ImmunoOncologyWorkflow Author: Alex Qin [aut], Alexander Nicholls [aut], Nicholas Robertson [aut], Nicolas Canete [aut], Elijah Willie [aut], Ellis Patrick [aut] (ORCID: ), SOMS Maintainer [aut, cre] Maintainer: SOMS Maintainer URL: https://github.com/SydneyBioX/spicyWorkflow VignetteBuilder: knitr BugReports: https://github.com/SydneyBioX/spicyWorkflow/issues git_url: https://git.bioconductor.org/packages/spicyWorkflow git_branch: devel git_last_commit: 517dc71 git_last_commit_date: 2025-04-15 Date/Publication: 2025-05-20 source.ver: src/contrib/spicyWorkflow_1.9.0.tar.gz vignettes: vignettes/spicyWorkflow/inst/doc/spicyWorkflow.html vignetteTitles: "Introduction to a spicy workflow" hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/spicyWorkflow/inst/doc/spicyWorkflow.R dependencyCount: 0 Package: variants Version: 1.33.0 Depends: R (>= 3.3.0), VariantAnnotation, org.Hs.eg.db, TxDb.Hsapiens.UCSC.hg19.knownGene, BSgenome.Hsapiens.UCSC.hg19, PolyPhen.Hsapiens.dbSNP131 Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 MD5sum: 06206ccb638c0f4693814767d07087ea NeedsCompilation: no Title: Annotating Genomic Variants Description: Read and write VCF files. Identify structural location of variants and compute amino acid coding changes for non-synonymous variants. Use SIFT and PolyPhen database packages to predict consequence of amino acid coding changes. biocViews: ImmunoOncologyWorkflow, AnnotationWorkflow, Workflow Author: Valerie Obenchain [aut], Martin Morgan [ctb], Bioconductor Package Maintainer [cre] Maintainer: Bioconductor Package Maintainer URL: https://bioconductor.org/help/workflows/variants/ VignetteBuilder: knitr git_url: https://git.bioconductor.org/packages/variants git_branch: devel git_last_commit: f0f6db1 git_last_commit_date: 2025-04-15 Date/Publication: 2025-04-22 source.ver: src/contrib/variants_1.33.0.tar.gz vignettes: vignettes/variants/inst/doc/Annotating_Genomic_Variants.html vignetteTitles: Annotating Genomic Variants hasREADME: FALSE hasNEWS: FALSE hasINSTALL: FALSE hasLICENSE: FALSE Rfiles: vignettes/variants/inst/doc/Annotating_Genomic_Variants.R dependencyCount: 82 Package: EGSEA123 Version: 1.33.0 Depends: R (>= 3.4.0), EGSEA (>= 1.5.2), limma (>= 3.49.2), edgeR, illuminaio Suggests: knitr, rmarkdown, BiocStyle License: Artistic-2.0 NeedsCompilation: no Title: Easy and efficient ensemble gene set testing with EGSEA Description: R package that supports the workflow article `Easy and efficient ensemble gene set testing with EGSEA', Alhamdoosh et al. (2017), F1000Research, 6:2010. biocViews: ImmunoOncologyWorkflow, Workflow, GeneExpressionWorkflow Author: Monther Alhamdoosh, Charity Law, Luyi Tian, Julie Sheridan, Milica Ng and Matthew Ritchie Maintainer: Matthew Ritchie URL: https://f1000research.com/articles/6-2010 VignetteBuilder: knitr Package: TCGAWorkflow Version: 1.33.0 Depends: R (>= 3.4.0) Imports: AnnotationHub, knitr, ELMER, biomaRt, BSgenome.Hsapiens.UCSC.hg19, circlize, c3net, ChIPseeker, ComplexHeatmap, ggpubr, clusterProfiler, downloader (>= 0.4), GenomicRanges, GenomeInfoDb, ggplot2, ggthemes, graphics, minet, motifStack, pathview, pbapply, parallel, rGADEM, pander, maftools, RTCGAToolbox, stringr, SummarizedExperiment, dplyr, plyr, matlab, MultiAssayExperiment, TCGAbiolinks, TCGAWorkflowData (>= 1.25.3), DT, gt License: Artistic-2.0 NeedsCompilation: no Title: TCGA Workflow Analyze cancer genomics and epigenomics data using Bioconductor packages Description: Biotechnological advances in sequencing have led to an explosion of publicly available data via large international consortia such as The Cancer Genome Atlas (TCGA), The Encyclopedia of DNA Elements (ENCODE), and The NIH Roadmap Epigenomics Mapping Consortium (Roadmap). These projects have provided unprecedented opportunities to interrogate the epigenome of cultured cancer cell lines as well as normal and tumor tissues with high genomic resolution. The Bioconductor project offers more than 1,000 open-source software and statistical packages to analyze high-throughput genomic data. However, most packages are designed for specific data types (e.g. expression, epigenetics, genomics) and there is no one comprehensive tool that provides a complete integrative analysis of the resources and data provided by all three public projects. A need to create an integration of these different analyses was recently proposed. In this workflow, we provide a series of biologically focused integrative analyses of different molecular data. We describe how to download, process and prepare TCGA data and by harnessing several key Bioconductor packages, we describe how to extract biologically meaningful genomic and epigenomic data. Using Roadmap and ENCODE data, we provide a work plan to identify biologically relevant functional epigenomic elements associated with cancer. To illustrate our workflow, we analyzed two types of brain tumors: low-grade glioma (LGG) versus high-grade glioma (glioblastoma multiform or GBM). biocViews: Workflow, ResourceQueryingWorkflow Author: Tiago Chedraoui Silva , Antonio Colaprico , Catharina Olsen , Fulvio D Angelo , Gianluca Bontempi , Michele Ceccarelli , Houtan Noushmehr Maintainer: Tiago Chedraoui Silva URL: https://f1000research.com/articles/5-1542/v2 VignetteBuilder: knitr BugReports: https://github.com/BioinformaticsFMRP/TCGAWorkflow/issues Package: maEndToEnd Version: 2.29.0 Depends: R (>= 3.5.0), Biobase, oligoClasses, ArrayExpress, pd.hugene.1.0.st.v1, hugene10sttranscriptcluster.db, oligo, arrayQualityMetrics, limma, topGO, ReactomePA, clusterProfiler, gplots, ggplot2, geneplotter, pheatmap, RColorBrewer, dplyr, tidyr, stringr, matrixStats, genefilter, openxlsx, Rgraphviz, enrichplot Suggests: BiocStyle, knitr, devtools, rmarkdown License: MIT + file LICENSE NeedsCompilation: no Title: An end to end workflow for differential gene expression using Affymetrix microarrays Description: In this article, we walk through an end-to-end Affymetrix microarray differential expression workflow using Bioconductor packages. This workflow is directly applicable to current "Gene" type arrays, e.g. the HuGene or MoGene arrays, but can easily be adapted to similar platforms. The data analyzed here is a typical clinical microarray data set that compares inflamed and non-inflamed colon tissue in two disease subtypes. For each disease, the differential gene expression between inflamed- and non-inflamed colon tissue was analyzed. We will start from the raw data CEL files, show how to import them into a Bioconductor ExpressionSet, perform quality control and normalization and finally differential gene expression (DE) analysis, followed by some enrichment analysis. biocViews: GeneExpressionWorkflow Author: Bernd Klaus [aut], Stefanie Reisenauer [aut, cre] Maintainer: Stefanie Reisenauer URL: https://www.bioconductor.org/help/workflows/ VignetteBuilder: knitr Package: SingscoreAMLMutations Version: 1.25.0 Title: ERROR Maintainer: ERROR Package: ExpHunterSuite Version: 1.17.0 Depends: R (>= 4.1.0) Imports: ReactomePA, limma, edgeR, NOISeq, biomaRt, topGO, diffcoexp, DT, ggplot2, stringr, WGCNA, dplyr, AnnotationDbi, BiocGenerics, enrichplot, rmarkdown, stats, Biobase, DESeq2, ROCR, data.table, knitr, magrittr, SummarizedExperiment, miRBaseVersions.db, grDevices, graphics, utils, BiocParallel, MKinfer, matrixStats, ggupset, rlang, plyr, tidyr, GO.db, Matrix, fastcluster, DOSE, heatmaply, EnhancedVolcano, ggrepel, clusterProfiler, GenomicRanges, GenomicFeatures, tximport, annotatr, ggridges, FactoInvestigate, FactoMineR Suggests: optparse, PerformanceAnalytics, naivebayes, reshape2, org.Hs.eg.db, org.Mm.eg.db, testthat (>= 3.0.0) License: MIT + file LICENSE Title: Package For The Comprehensive Analysis Of Transcriptomic Data Description: The ExpHunterSuite implements a comprehensive protocol for the analysis of transcriptional data using established *R* packages and combining their results. It covers all key steps in DEG detection, CEG detection and functional analysis for RNA-seq data. It has been implemented as an R package containing functions that can be run interactively. In addition, it also contains scripts that wrap the functions and can be run directly from the command line. biocViews: GeneExpressionWorkflow Author: James Perkins [aut, cre] (ORCID: ), Pedro Seoane Zonjic [aut] (ORCID: ), Fernando Moreno Jabato [aut] (ORCID: ), José Córdoba Caballero [aut] (ORCID: ), Elena Rojano Rivera [aut] (ORCID: ), Rocio Bautista Moreno [aut] (ORCID: ), M. Gonzalo Claros [aut] (ORCID: ), Isabel Gonzalez Gayte [aut], Juan Antonio García Ranea [aut] (ORCID: ) Maintainer: James Perkins VignetteBuilder: knitr Package: GeoMxWorkflows Version: 1.15.0 Depends: R (>= 4.0), NanoStringNCTools, GeomxTools Imports: Biobase, S4Vectors, rjson, readxl, EnvStats, dplyr, reshape2, methods, utils, stats, data.table, outliers, BiocGenerics, ggplot2, ggrepel, ggforce, cowplot, scales, umap, Rtsne, pheatmap, BiocStyle, networkD3 Suggests: rmarkdown, knitr License: MIT Title: GeoMx Digital Spatial Profiler (DSP) data analysis workflows Description: Workflows for use with NanoString Technologies GeoMx Technology. Package provides bioconductor focused workflows for leveraging existing packages (e.g. GeomxTools) to process, QC, and analyze the data. biocViews: GeneExpressionWorkflow, ImmunoOncologyWorkflow, SpatialWorkflow Author: Maddy Griswold [cre, aut], Jason Reeves [aut], Prajan Divakar [aut], Nicole Ortogero [aut], Zhi Yang [aut], Stephanie Zimmerman [aut], Rona Vitancol [aut], David Henderson [aut] Maintainer: Maddy Griswold VignetteBuilder: knitr Package: seqpac Version: 1.9.0 Depends: R (>= 4.2.0) Imports: Biostrings (>= 2.46.0), foreach (>= 1.5.1), GenomicRanges (>= 1.30.3), Rbowtie (>= 1.18.0), ShortRead (>= 1.36.1), tibble (>= 3.1.2), BiocParallel (>= 1.12.0), cowplot (>= 0.9.4), data.table (>= 1.14.0), digest (>= 0.6.27), doParallel (>= 1.0.16), dplyr (>= 1.0.6), factoextra (>= 1.0.7), FactoMineR (>= 1.41), ggplot2 (>= 3.3.3), IRanges (>= 2.12.0), parallel (>= 3.4.4), reshape2 (>= 1.4.4), rtracklayer (>= 1.38.3), stringr (>= 1.4.0), stats (>= 3.4.4), methods, S4Vectors Suggests: benchmarkme (>= 0.6.0), DESeq2 (>= 1.18.1), GenomeInfoDb (>= 1.14.0), gginnards (>= 0.0.2), qqman (>= 0.1.8), rmarkdown, BiocStyle, knitr, testthat, UpSetR (>= 1.4.0), venneuler, R.utils, bigreadr, readr, vroom License: GPL-3 Title: Seqpac: A Framework for smallRNA analysis in R using Sequence-Based Counts Description: Seqpac provides functions and workflows for analysis of short sequenced reads. It was originally developed for small RNA analysis, but can be implemented on any sequencing raw data (provided as a fastq-file), where the unit of measurement is counts of unique sequences. The core of the seqpac workflow is the generation and subsequence analysis/visualization of a standardized object called PAC. Using an innovative targeting system, Seqpac process, analyze and visualize sample or sequence group differences using the PAC object. A PAC object in its most basic form is a list containing three types of data frames. - Phenotype table (P): Sample names (rows) with associated metadata (columns) e.g. treatment. - Annotation table (A): Unique sequences (rows) with annotation (columns), eg. reference alignments. - Counts table (C): Counts of unique sequences (rows) for each sample (columns). The PAC-object follows the rule: - Row names in P must be identical with column names in C. - Row names in A must be identical with row names in C. Thus P and A describes the columns and rows in C, respectively. The targeting system, will either target specific samples in P (pheno_target) or sequences in A (anno_target) and group them according to a target column in P and A, respectively (see vignettes for more details). biocViews: Workflow, BasicWorkflow, GeneExpressionWorkflow, EpigeneticsWorkflow, AnnotationWorkflow Author: Daniel Natt [aut, cre, fnd], Lovisa Örkenby [ctb], Signe Skog [ctb], Anita Öst [aut, fnd] Maintainer: Daniel Natt URL: https://github.com/Danis102/seqpac VignetteBuilder: knitr BugReports: https://github.com/Danis102/seqpac/issues