############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.22-bioc/R/bin/R CMD build --keep-empty-dirs --no-resave-data --md5 TAPseq ### ############################################################################## ############################################################################## * checking for file ‘TAPseq/DESCRIPTION’ ... OK * preparing ‘TAPseq’: * checking DESCRIPTION meta-information ... OK * installing the package to build vignettes * creating vignettes ... ERROR --- re-building ‘tapseq_primer_design.Rmd’ using rmarkdown --- finished re-building ‘tapseq_primer_design.Rmd’ --- re-building ‘tapseq_target_genes.Rmd’ using rmarkdown Performing log-normalization 0% 10 20 30 40 50 60 70 80 90 100% [----|----|----|----|----|----|----|----|----|----| **************************************************| Quitting from tapseq_target_genes.Rmd:54-59 [unnamed-chunk-3] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Error: ! The `slot` argument of `GetAssayData()` was deprecated in SeuratObject 5.0.0 and is now defunct. ℹ Please use the `layer` argument instead. --- Backtrace: ▆ 1. └─TAPseq::selectTargetGenes(bone_marrow_genex) 2. ├─Seurat::GetAssayData(object, slot = "counts", assay = "RNA") 3. └─SeuratObject:::GetAssayData.Seurat(object, slot = "counts", assay = "RNA") 4. └─SeuratObject::.Deprecate(...) 5. └─lifecycle::deprecate_stop(...) 6. └─lifecycle:::deprecate_stop0(msg) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Error: processing vignette 'tapseq_target_genes.Rmd' failed with diagnostics: The `slot` argument of `GetAssayData()` was deprecated in SeuratObject 5.0.0 and is now defunct. ℹ Please use the `layer` argument instead. --- failed re-building ‘tapseq_target_genes.Rmd’ SUMMARY: processing the following file failed: ‘tapseq_target_genes.Rmd’ Error: Vignette re-building failed. Execution halted